FORMULASI BIOINSEKTISIDA DARI EKSTRAK KLOROFORM BATANG TUMBUHAN BAKAU HITAM (Rhizophora mucronata Lamk.) (BIOINSECTICIDE FORMULATIONS OF CHLOROFORM EXTRACTS OF MANGROVE PLANT (Rhizophora mucronata Lamk.))

  • AZMI LUDYAHANTORO Jurusan Kimia FMIPA-Universitas Negeri Surabaya

Abstract

Abstrak. Penelitian ini bertujuan untuk mengetahui hasil uji bioaktifitas pengembangan formula ekstrak klorofom batang tumbuhan bakau hitam (EKBH) dan efektifitas hasil pengujian semilapang formula EKBH. Pengembangan formula EKBH menggunakan bahan aktif ekstrak kloroform batang tumbuhan bakau hitam (EKBH) dan ekstrak metanol biji mimba (EMBM) dengan perbandingan konsentrasi 2:1. Pengujian bioaktivitas formula EKBH terhadap larva Spodoptera littura instar II menggunakan metode semprot agar terjadinya racun kontak dan racun perut dengan konsentrasi 0; 200; 400; 800; 1600; 3200 dan 6400 mg/L. Pengujian semilapang efikasi formula EKBH (2:1) disusun dalam Rancangan Acak Kelompok dengan 4 kali ulangan pada konsentrasi 0; 2; 4; 6; 8 EC dan Organeem (insektisida pembanding). Hasil penelitian menunjukkan bahwa pada uji formula EKBH (2:1) didapatkan nilai LC50 sebesar 3907,1 mg/L, Nilai EI pengujian semilapang menunjukkan formula EKBH 8,0 EC adalah 51,06 % tidak berbeda jauh atau hampir sama dengan pembanding Organeem sebesar 38,29 % sehingga formula EKBH 8,0 EC dapat dikatakan efektif.

Kata kunci : bioinsektisida, formulasi, Rhizophora mucronata Lamk.

Abstract. The aim of this research was to know the results of bioactivity test toward formulae of chloroform extracts of mangrove plant (EKBH) and effectiveness tested results of the semi-field EKBH formula. Formulate EKBH was using the chloroform extract active subtance of plant black mangrove stems (EKBH) and methanol extract of Mimba seed (EMBM) with a concentration ratio of 2:1. Bioactivity testing of EKBH formula to the 2nd instar larvae of Spodoptera littura using the spray method that occur contact poison and stomach poison with concentrations of 0; 200; 400; 800; 1600; 3200 and 6400 mg/L. Semi-field testing of the efficacy EKBH formula (2:1) arranged in a randomized block design with four replications at a concentration of 0, 2, 4, 6; 8  EC and Organeem (insecticide comparison). The results showed that the ​​ test EKBH formula (2:1) obtained LC50 values ​​of 3907.1 mg/L, semi-field testing EI value indicated EKBH 8.0 EC formula was 51,06% did not differ much or nearly equal to the comparison Organeem by 38.29% so that the EKBH formula 8.0 EC may be effective.

Key words: bioinsecticide, formulation, Rhizophora mucronata Lamk.

Published
2012-05-31
Section
Articles
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