Media Alternatif untuk Pertumbuhan Sel Midgut Spodoptera litura

Abstract

Cell culture is process when viable cell propagated in vitro in the medium. Midgut cell culture of S. litura is very useful as hosts for SpltMNPV virus that can control S. litura. This study aimed to determine the increase in the number of S. litura midgut cells and long incubation time of S. litura midgut cells in RPMI 1640, DMEM, and mixture of RPMI 1640 medium and DMEM as alternative medium with initial inoculum 4.0 x 10⁵ cell/ml. This research used Completely Randomized Design (CRD) with 4 treatments and 3 repetitions so that there are 12 treatment unit. Data was analyzed using ANOVA (Analysis of Variance). Further tests are LSD (Least Significant Difference). The results showed that the highest increase of the number of S. litura midgut cells was obtained in Grace's medium (control) with 23125 times inoculum, the mixture of RPMI 1640 and DMEM medium was  21950 times inoculum, RPMI 1640 medium was  11725 times, DMEM medium was  10400 times inoculum. Spodoptera litura midgut cells form a monolayer in a 24-hour incubation in Grace's medium, the mixture of RPMI 1640 and DMEM medium, and DMEM, whereas the monolayer formation in RPMI 1640 medium is in a 48-hour incubation. The result showed that the mixture of RPMI 1640 and DMEM medium is an .optimum alternative medium that support the growth of Spodoptera litura midgut cell.