Characterization and Effectiveness Test Gel of Aloe vera Combination Chitosan as Staphylococcus aureus Antibacterial

  • Sari Edi Cahyaningrum Universitas Negeri Surabaya
  • Perintis Gita Susanti Universitas Negeri Surabaya

Abstract

The study about development synthesis gel of Aloe vera combination chitosan as Staphylococcus aureus antibacterial has been done. This study has the aim to determine the effect of composition comparison of Aloe vera with chitosan on the characterization and effectiveness of Staphylococcus aureus antibacterial as a candidate for wound healing. The synthesis the gel of Aloe vera combination chitosan was made by mixing of Aloe vera and chitosan solution with weight variated 2:1; 1:1; and 1:2 (AvK1, AvK2, and AvK3), and addition of Na-CMC, glycerin, and propylene glycol, which the gel was neutralized until it reached pH 6,5–7,0. The gel preparation were characterized by organoleptic, measuring viscosity, functional groups identification, and effectiveness test of Staphylococcus aureus antibacterial by diffusion method. The results were shown based organoleptic observations by color, smell, and texture. The lowest viscosity measurement characterization was showed by AvK3 gel, which was 13.0412 mPa.s. The result functional groups identification of all gel preparations was indicated the presence of O–H and N–H stretching vibrations, C=O stretching vibrations, C–H bending vibrations, and C–O stretching vibrations. Staphylococcus aureus antibacterial test from gel of Aloe vera combination chitosan with various variations were shown effective result, which the strongest was shown by AvK1 gel with an inhibition zone of 26 mm.

 

Key words: chitosan, Aloe vera, antibacterial, Staphylococcus aureus

References

Saputro, I. D. 2014. Dasar-Dasar Biomolekuler Penyembuhan Luka. Surabaya: Global Persada Press.

Dewi, R. and Marniza, E. 2019. Aktivitas Antibakteri Gel Lidah Buaya terhadap Staphylococcus aureus, Jurnal Saintek Lahan Kering, 2(2622), pp. 61–62.

Gibson, J. M. 1996. Mikrobiologi dan Patologi Modern untuk Perawat. Jakarta: EGC.

Firdaus, T. 2014. Efektivitas Ekstrak Bawang Dayak (Eleutherine palmifolia) dalam Menghambat Pertumbuhan Bakteri Staphylococcus aureus. Jakarta.

Aponno, J. V, Yamlean, P. V. Y. and Supriati, H. S. 2014. Uji Efektivitas Sediaan Gel Ekstrak Etanol Daun Jambu Biji (Psidium guajava Linn) terhadap Penyembuhan Luka yang Terinfeksi Bakteri Staphylococcus aureus pada Kelinci (Orytolagus cuniculus), Jurnal Ilmiah Farmasi, 3(3), pp. 279–286.

Jawetz, E., Melnick, J. and Adelberg’s, E. A. 2005. Mikrobiologi Kedokteran. 25th edn. Jakarta: Salemba Medika.

Kantawong, F. et al. 2016. Mucus of Achatina fulica Stimulates Mineralization and Inflammatory Response in Dental Pulp Cells, Turkish Journal of Biology, 40(2), pp. 353–359. doi: 10.3906/biy-1505-29.

Johnson, K. E. and Wilgus, T. A. 2014. Vascular Endothelial Growth Factor and Angiogenesis in the Regulation of Cutaneous Wound Repair, Adv Wound Care, 3(10), pp. 647–661. doi: 10.1089/wound.2013.0517.

Ray, S. S. 2013. Environmentally Friendly Polymer Nanocomposites. Cambridge: Woodhead Publishing.

Bano, I. et al. 2017. Chitosan: A Potential Biopolymer for Wound Management, International Journal of Biological Macromolecules, 102, pp. 380–383. doi: 10.1016/j.ijbiomac.2017.04.047.

Nikmah, F. and Cahyaningrum, S. E. 2014. Alginat Preparation and Characterization of Chitosan-Ca–Alginate Membrane, Unesa Journal of Chemistry, 3(1), pp. 55–59.

Pratiwi, R. 2014. Manfaat Kitin dan Kitosan bagi Kehidupan Manusia, Oseana, XXXIX(1), pp. 35–43. Available at: http://oseanografi.lipi.go.id/dokumen/os_xxxix_1_2014-4.pdf.

Khan, M. A. and Mujahid, M. 2019. A Review on Recent Advances in Chitosan Based Composite for Hemostatic Dressings, International Journal of Biological Macromolecules, 124, pp. 138–147. doi: 10.1016/j.ijbiomac.2018.11.045.

Nurainy, F., Rizal, S. and Yudiantoro. 2008. Pengaruh Konsentrasi Kitosan terhadap Aktivitas Antibakteri dengan Metode Difusi, Jurnal Teknologi Industri dan Hasil Pertanian, 13(2), pp. 117–125.

Matica, M. A., Aachmann, F. L., Tøndervik, A., Sletta, H., and Ostafe, V. 2019. Chitosan as a Wound Dressing Starting Material : Antimicrobial Properties and Mode of Action, Int. J. Mol. Sci., (1), pp. 1–33.

Bashir, A. et al. 2011. Comparative Study of Antimicrobial Activities of Aloe Vera Extracts and Antibiotics Against Isolates from Skin Infections, African Journal of Biotechnology, 10(19), pp. 3835–3840. doi: 10.4314/ajb.v10i19.

Furnawanthi, I. 2007. Khasiat dan Manfaat Lidah Buaya. 8th edn. Jakarta: Agro Media Pustaka.

Hu, Y., Xu, J. and Hu, Q. 2003. Evaluation of Antioxidant Potential of Aloe vera (Aloe barbadensis Miller) Extracts, Journal of Agricultural and Food Chemistry, 51(26), pp. 7788–7791. doi: 10.1021/jf034255i.

Maulani, I. 2015. Perbandingan Pemberian Gel Lidah Buaya (Aloe vera L.) dan Povidone Iodine terhadap Waktu Penyembuhan Luka Iris (Vulnus scissum) pada Mencit (Mus musculus) Jantan Galur Wistar. Palembang.

Sharma, K., Mittal, A. and Chauhan, N. 2016. Aloe vera as Penetration Enhancer, Int. J. Drug Dev. & Res., 7(January-March 2015), pp. 280–285.

Suhartono, M., Sularsih and Nafi’ah. 2018. Perbedaan Pengaruh Aplikasi Gel Kombinasi Kitosan Berat Molekul Tinggi dan Rendah dengan Ekstrak Lidah Buaya (Aloe vera) Terhadap Kepadatan Kolagen pada Proses Penyembuhan Ulkus Traumatikus, Jurnal Kedokteran Gigi, 12(1), pp. 60–71.

Silva, S. S. et al. 2013. Effect of Crosslinking in Chitosan/Aloe Vera-Based Membranes for Biomedical Applications, Carbohydrate Polymers, pp. 1–31. doi: 10.1016/j.carbpol.2013.06.022.

Mondal, I. H. and Saha, J. 2019. Antimicrobial, UV Resistant and Thermal Comfort Properties of Chitosan-and Aloe vera-Modified Cotton Woven Fabric, Journal of Polymers and the Environment, 0(0), p. 0. doi: 10.1007/s10924-018-1354-9.

Agustina, L., Shoviantari, F. and Aditya, D. 2019. Pengaruh Konsentrasi Mucin (Achatina Fulica) terhadap Kualitas Fisik dan Stabilitas Mucin Gel, Jurnal Wiyata, 6(1), pp. 31–39.

Imanto, T., Prasetiawan, R. and Wikantyasning, E. R. 2019. Formulasi dan Karakterisasi Sediaan Nanoemulgel Serbuk Lidah Buaya (Aloe Vera L.), Jurnal Farmasi Indonesia, 16(1), pp. 28–37.

Waty, H. R. 2012. Modifikasi Kitosan pada Aplikasi Plester Luka Berbasis Kitosan (Chitoplast) sebagai Transdermal Patch Antibakteri. Bogor.

Susanti, R., Yuniastuti, A. and Iswari, R. S. 2012. Aktivitas Reactive Oxygen Species Makrofag akibat Stimulasi Gel Lidah Buaya pada Infeksi Salmonella typhimurium, Jurnal MIPA, 35(1), pp. 1–10.

Fitrian, A., Bashori, A. and Sudiana, I. K. 2018. Efek Angiogenesis Gel Ekstrak Daun Lamtoro (Leucaena leucocephala) pada Luka Insisi Tikus, Jurnal Biosains Pascasarjana, 20(1).

Aswarita, R. 2013. Interaksi Ekstrak Daun Lidah Buaya (Aloe vera L.) dan Daun Jambu Biji (Psidium guajava L.) terhadap Daya Hambat Escherichia coli secara In Vitro, Jurnal EduBio Tropika, 1(2), pp. 115–120.

Adnan, J. 2016. Formulasi Gel Ekstrak Daun Beluntas (Pluceaindica Less) dengan Na-CMC sebagai Basis Gel, Journal of Pharmaceutical Science and Herbal Technology, 1(1), pp. 41–44.

Usman, Y. 2018. Perbandingan Uji Stabilitas dan Aktivitas Gel Lidah Buaya (Aloe vera L.) pada Basis Na-CMC dan Karbopol, Jurnal Ilmiah Kesehatan Diagnosis, 12(6), pp. 621–625.

Shanti, P. C. 2019. Formulasi dan Uji Aktivitas Antioksidan Emulgel Minyak Atsiri Bunga Cengkeh menggunakan Metode (1,1-Difenil-2-Pikrilhidrazil) DPPH. Malang.

Garg, A., Aggarwal, D., Garg, S., and Singla, A. K. 2002. Spreading of Semisolid Formulations An Update, Pharm. Technol., September, pp. 84–105.

Dewan Standarisasi Nasional. 1996. SNI 16-4399-1996 Tentang Sediaan Tabir Surya. Jakarta: Dewan Standarisasi Nasional.

Pratiwi, S. T. 2008. Mikrobiologi Farmasi. Jakarta: Penerbit Erlangga.

Davis, W. W. and Stout, T. R. 1971. Disc Plate Methods of Microbiological Antibiotic Assay, Microbiology, pp. 659–665.

Darsana, I. G. O., Besung, I. N. K. and Mahatmi, H. 2012. Potensi Daun Binahong (Anredera cordifolia) dalam Menghambat Pertumbuhan Bakteri Escherichia coli secara In Vitro, Indonesia Medicus Veterinus, 1(3), pp. 337–345.

Kibret, B. et al. 2018. Comparative Study of the Antibacterial Activity of Leaves of Croton macrostachyus and Aloe vera’, Advances in Life Science and Technology, 54, pp. 22–28.

Published
2022-02-11
Section
Articles
Abstract Views: 358
PDF Downloads: 662